Table 1.
Concentrations (nM) of low-molecular-weight thiols in blood and tissues of wild type (WT) and Nrf2 KO mice (mean ± SD, n = 5 per group).
| WT mice | Nrf2 KO mice | p value (versus WT mice) | |
|---|---|---|---|
| Heart | |||
| Total GSH (nM) | 144.3 ± 53.11 | 91.52 ± 12.06 | =0.0620 (↓ ns) |
| GSH (nM) | 143.7 ± 52.77 | 90.77 ± 11.99 | =0.0602 (↓ ns) |
| GSSG (nM) | 0.6263 ± 0.427 | 0.7531 ± 0.352 | 0.6225 |
| GSH/GSSG | 335.0 ± 217.9 | 140.2 ± 52.51 | =0.0887 (↓ ns) |
| Cys (nM) | 16.38 ± 6.976 | 8.99 ± 1.804 | =0.051 (↓ ns) |
| HCys (nM) | 0.6387 ± 0.1949 | 0.4231 ± 0.06714 | ↓∗0.0475 |
| GluCys (nM) | 0.2066 ± 0.03208 | 0.1852 ± 0.02159 | 0.2506 |
| Sulfide (nM) | 45.9 ± 22.1 | 28.56 ± 6.597 | 0.1301 |
| Aorta | |||
| Total GSH (nM) | 217.2 ± 73.95 | 105.9 ± 21.91 | ↓∗0.0121 |
| GSH (nM) | 216.4 ± 73.54 | 105.2 ± 22.2 | ↓∗0.0119 |
| GSSG (nM) | 1.099 ± 0.2598 | 0.7146 ± 0.4136 | 0.1517 |
| GSH/GSSG | 224.8 ± 58.4 | 186.9 ± 101.2 | 0.5308 |
| Cys (nM) | 54.18 ± 12.78 | 18.15 ± 3.143 | ↓∗∗∗0.0003 |
| HCys (nM) | 2.324 ± 0.3268 | 3.226 ± 2.024 | 0.3539 |
| GluCys (nM) | 0.9574 ± 0.1772 | 0.6319 ± 0.1166 | ↓∗∗0.0089 |
| Sulfide (nM) | 21.51 ± 5.773 | 16.78 ± 5.570 | 0.2237 |
| Liver | |||
| Total GSH (nM) | 8097 ± 833.6 | 4742 ± 2042 | ↓∗∗0.0094 |
| GSH (nM) | 8028 ± 814.8 | 4592 ± 2025 | ↓∗∗0.0078 |
| GSSG (nM) | 68.61 ± 27.77 | 150.6 ± 43.52 | ↑∗∗0.0075 |
| GSH/GSSG | 127.2 ± 31.83 | 31.55 ± 15.14 | ↓∗∗∗0.0003 |
| Cys (nM) | 204.8 ± 16.85 | 179.1 ± 52.44 | 0.3272 |
| HCys (nM) | 39.12 ± 7.435 | 18.18 ± 6.563 | ↓∗∗0.0015 |
| GluCys (nM) | 0.7695 ± 0.04889 | 0.5463 ± 0.1343 | ↓∗∗0.0082 |
| CysGly (nM) | 96.75 ± 16.4 | 62.13 ± 21.8 | ↓∗0.0219 |
| Sulfide (nM) | 13.19 ± 2.683 | 15.17 ± 4.521 | 0.4229 |
| Plasma | |||
| Total GSH (nM) | 27,144 ± 8464 | 16,594 ± 3540 | ↓∗0.0331 |
| GSH (nM) | 26,456 ± 8364 | 16,194 ± 3482 | ↓∗0.0351 |
| GSSG (nM) | 688.3 ± 171 | 399.9 ± 93.14 | ↓∗0.0107 |
| GSH/GSSG | 39.04 ± 10.17 | 41.28 ± 7.372 | 0.7005 |
| Cys (nM) | 11,900 ± 2432 | 8207 ± 1249 | ↓∗0.0165 |
| HCys (nM) | 337 ± 159 | 209.2 ± 35.86 | 0.1177 |
| GluCys (nM) | 668.8 ± 140.9 | 320.1 ± 83.34 | ↓∗∗0.0014 |
| CysGly (nM) | 725 ± 186 | 1422 ± 728.2 | = 0.0751 (↓ ns) |
| Sulfide | 332.1 ± 46.77 | 457.7 ± 386.6 | 0.4912 |
| Erythrocytes | |||
| Total GSH (nM) | 3.94∗106 ± 0.52∗106 | 3.77∗106 ± 0.30∗106 | 0.5570 |
| GSH (nM) | 3.93∗106 ± 0.52∗106 | 3.77∗106 ± 0.30∗106 | 0.5583 |
| GSSG (nM) | 1058 ± 103.7 | 781.5 ± 153.3 | ↓∗0.0103 |
| GSH/GSSG | 3766 ± 726.2 | 4946 ± 841 | ↑∗0.0449 |
| Cys (nM) | 26,254 ± 10,655 | 18,796 ± 2980 | 0.1702 |
| HCys (nM) | 754.3 ± 317.9 | 550.8 ± 104.8 | 0.2109 |
| GluCys (nM) | 286.7 ± 118.3 | 202.7 ± 44.78 | 0.1757 |
| CysGly (nM) | 11,488 ± 5053 | 11,772 ± 3183 | 0.9178 |
| Sulfide (nM) | 387.3 ± 69.59 | 490.7 ± 98.66 | 0.0917 |
ns - not significant; ↓ - decreased; ↑ - increased; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.