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. 2017 Nov 7;20(3):284–289. doi: 10.4103/aja.aja_46_17

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Copyright: © The Author(s)(2017)

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Confirmation of HBV s and x gene transcription in sperm cells. (a) Sperm cells in test group A were obtained from patients with chronic HBV infection, and those in control Group A were obtained from healthy donors. (b) Sperm cells were obtained from healthy donors and then divided into test Group B, which were transfected with a plasmid containing the full-length HBV genome, and control Group B, which were nontransfected. M: marker DL 50~500; Lane 1: GAPDH in test Group A and B; Lane 2: s gene in test Group A and B; Lane 3: x gene in test Group A and B; Lanes 4 and 9: minus reverse transcriptase control (–RT); Lanes 5 and 10: minus template control (–T). Lane 6: GAPDH in control Group A and B; Lane 7: amplification of s gene in control Group A and B; Lane 8: amplification of x gene in control Group A and B. Positive bands for GAPDH were observed in test Group A and B as well as in control Group A and B; positive bands for s and x genes were observed only in test Group A and B and not in control Group A and B or in –RT and –T, suggesting that s and x gene transcriptional activity occurs in sperm cells. GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HBV: hepatitis B virus.