Figure 2.

Arginase activity (▲) and protein quantity (■) curves after gel chromatography of serum proteins in healthy individuals, breast cancer patients, and patients after chemotherapy (stage II, n = 7, p < 0.05). (a) Healthy, (b) breast cancer, and (c) chemotherapy. The enzyme obtained after several steps of purification (see Materials and Methods; 45 mg, 51 mg, and 52 mg protein, resp., for healthy individuals, breast cancer patients, and patients after chemotherapy) was applied to a Sephadex G-150 column (2 × 40 cm).