Table 2.

Larvicidal activity of Zanthoxylum acanthopodium essential oil, estragole and eucalyptol against fourth instar Anopheles sinensis larvae

Compounds	Concentration (mg/L)	24 h mortality (%)	LC50 (mg/L) (95% CI)	LC90 (mg/L) (95% CI)	Slope ± S.E.	Chisquare (df)
Essential oil	25	17	49.02 (44.15–49.41)	125.18 (105.90–141.74)	3.29 ± 0.28	2.835 (3)a
	50	54
	75	68
	100	83
	125	94
	150	100
Estragole	25	28	41.67 (29.48–52.07)	107.89 (82.90–178.83)	3.10 ± 0.28	5.994 (3)a
	50	57
	75	72
	100	89
	125	97
	150	100
Eucalyptol	25	26	45.49 (33.24–56.33)	124.95 (94.78–213.84)	2.92 ± 0.27	5.329 (3)a
	50	51
	75	69
	100	83
	125	95
	150	100
DMSO		1 ± 0.45

Each datum represents the mean of five replicates, each set up with 20 individuals (n = 100)

LC values are considered significantly different when 95% CI fail to overlap

95% CI confidence interval at 95% confidence level

^aThe significance level is more than 0.05. A heterogeneity factor is used in the calculation of confidence limits