Fig. 2. (a) Fluorescent spectra of FCANB reacted with/without PYP or cationic PYP mutants. [Probe]/[PYP] = 2 μM/3 μM. (b) Time course of fluorescence intensity of FCANB at 522 nm with PYP-tags. [Probe]/[PYP] = 6 μM/5 μM. All the measurements were conducted at 37 °C using assay buffer of pH 7.4 including 20 mM HEPES and 150 mM NaCl.
