Fig. 3.

Acteoside identification/quantification in mouse plasma and grafted tumors with the use of UHPLC-MS analyses. (A) Calibration curve of the analyte/IS vs. increasing concentrations of acteoside. (B) Representative LC-MS chromatogram of acteoside in mice plasma. (C) Representative MS spectrum of acteoside in treated mice plasma (C₁) and relative quantification (C₂) after OR or IP administration of the compound. (D) Quantification of acteoside in excised tumors after OR or IP administration of the compound in mice. Bars, ± SD (n ≥ 2). * , P < 0.05; * *, P < 0.01.