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. Author manuscript; available in PMC: 2018 Oct 11.
Published in final edited form as: Cell Host Microbe. 2017 Oct 11;22(4):460–470.e5. doi: 10.1016/j.chom.2017.09.002

Figure 3. Hap1 cells reconstituted with different SPCA1 isoforms and point mutations.

Figure 3

CRISPR-generated WT and KO clones of SPCA1 were reconstituted with (A and C) SPCA1 isoforms 1A-F and (B and D) isoform 1D bearing Hailey-Hailey disease-derived point mutations (G309C and D742Y) important in Ca2+-ion binding. Hap1 cells, un-reconstituted and reconstituted clones were infected with (A and B) RSV at a MOI of 0.1 and (C and D) hPIV-3-GFP at a MOI of 0.01. Cells, harvested (A and B) at 72 hpi and (C and D) at 48 hpi, were analyzed by flow cytometry and plotted as a percentage of GFP positive cells. See also Figures S1 and S2.