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. Author manuscript; available in PMC: 2018 May 15.
Published in final edited form as: J Biomed Mater Res B Appl Biomater. 2014 Nov 12;103(7):1381–1389. doi: 10.1002/jbm.b.33310

Figure 4. Tetracycline Resistant E. coli and S. aureus are prevented from colonizing Ti-TET rods.

Figure 4

Control rods (A) and Ti-TET rods (B) were incubated with TETR E. coli (8 a generous gift of Dr. Lars Hansen) for 4 hrs, the bacteria were then stained using the LIVE/DEAD® BacLight™ Viability Kit and imaged using confocal microscopy. Note that while colony formation of these bacteria is fairly low on control surfaces, it is completely inhibited on the Ti-TET surface. In parallel, S. aureus was incubated with Control (C) and Ti-TET rods for 4 h. The bacteria were then stained using the Live/Dead® BacLight™ Viability Kit and imaged using confocal microscopy. Control rods were abundantly colonized while the Ti-TET rods showed diminished, albeit not absent, S. aureus colonization. Fluorescence is presented as an intensity map, with signal intensity legend shown in D.