Fig. 3.
LncMAPK6 was required for liver TIC self-renewal. (a) LncMAPK6 depleted cells were generated through CRISPRi strategy, and sphere formation assay was performed to detect liver TIC self-renewal. LncMAPK6 depletion efficiency was examined by Northern blot (left). Typical photos and the ratios of sphere-initiating cells were shown. (b) 10, 1 × 102, 1 × 103, 1 × 104 and 1 × 105 lncMAPK6 depleted cells were used for 3 months’ tumor formation. Tumor initiating cell ratios were analyzed through extreme limiting dilution analysis (right panels). CI, confidence interval. (c) Transwell assay was performed using lncMAPK6 silenced cells, and representative images and cell numbers were shown. (d, e) LncMAPK6 antisense oligo (ASO) treated cells were utilized for sphere formation (d) and invasion assay (e), and the typical images were shown. (f) LncMAPK6 overexpressed cells were established by CRISPRa strategy, and enhanced sphere formation capacity was found in lncMAPK6 overexpressing cells. (g) 10, 1 × 102, 1 × 103, 1 × 104 and 1 × 105 lncMAPK6 overexpressed cells were used for 3 months’ tumor initiation