Table 1.

Larvicidal activity of Aqueous, 1 mM silver nitrate solution and synthesized AgNPs of L. aspera leaf extract against fourth instar larvae of A. stephensi, A. aegypti and C. quinquefasciatus.

Extract	Species	Con (mg/mL)	Mortality% ± SD	LC50 ± SE (UCL–LCL) (mg/mL)	LC90 ± SE (UCL–LCL) (mg/mL)	χ2 (df = 4)
Aqueous	A. stephensi	100	88 ± 1.82	26.08 ± 2.15 (30.31–21.85)	147.61 ± 26.61 (134.89–67.95)	4.96
		50	68 ± 1.67
		25	41 ± 1.22
		12.5	28 ± 0.84
		6.25	19 ± 0.71
	A. aegypti	100	90 ± 2.17	17.16 ± 1.52 (20.15–14.18)	101.42 ± 17.07 (134.89–67.95)	4.16
		50	73 ± 2.51
		25	59 ± 3.03
		12.5	40 ± 1.48
		6.25	26 ± 1.34
	C. quinquefasciatus	100	85 ± 2.05	33.68 ± 3.01 (39.59–27.78)	207 ± 44.08 (294.08–121.28)	9.7
		50	57 ± 1.79
		25	39 ± 0.89
		12.5	23 ± 1.48
		6.25	17 ± 2.05
Silver nitrate	(1 mM solution)	10	65 ± 1.67	21.25 ± 5.09 (32.19–12.38)	73.49 ± 35.72 (142.57–43.42)	4.99
		8	51 ± 2.51
		6	32 ± 4.47
		4	18 ± 1.30
		2	08 ± 0.87
AgNPs	A. aegypti	10	100 ± 0.00	4.02 ± 0.21 (4.44–3.59)	11.22 ± 9.91 (13.16–9.28)	32.5
		8	80 ± 1.55
		6	54 ± 1.30
		4	41 ± 0.84
		2	28 ± 1.00
	A. stephensi	10	100 ± 0.00	4.69 ± 0.22 (5.13–4.57)	12.09 ± 1.04 (14.13–10.05)	40.7
		8	75 ± 1.10
		6	45 ± 1.67
		4	34 ± 1.66
		2	21 ± 1.00
	C. quinquefasciatus	10	100 ± 0.00	5.06 ± 0.22 (5.51–4.61)	12.74 ± 1.10 (14.90–10.58)	49.1
		8	72 ± 1.87
		6	39 ± 0.89
		4	29 ± 1.14
		2	19 ± 0.84