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PCR of the ITS2 region of members of the An. funestus group and three examples of the Shingwedzi (South Africa) specimens. PCR amplification from the specimens produced an amplicon of ~ 550 bp, which is similar to the An. rivulorum size fragment. Lanes 1 and 11: 100 bp DNA ladder; lane 2: An. funestus; lane 3: An. leesoni; lane 4: An. parensis; lane 5: An. rivulorum; lane 6: An. vaneedeni; lane 7: negative control; lanes 8–10: three specimens from Shingwedzi
