Fig. 2.
Uric acid does not affect dHL-60 cell viability. (A) dHL-60 cells were incubated with PA14 (MOI 1:10) for one or two hours at 37 °C. Dead cells were stained with PI and the fluorescence (λex = 535 nm, λem = 620 nm) was measured by flow cytometry. Staurosporine (St) was used as a positive control. (B) Cytotoxicity was measured by lactate dehydrogenase (LDH) activity in supernatants of dHL-60 incubated or not with PA14 (MOI 1:10) and uric acid (UA) for 3 h at 37 °C. LDH activity is presented as the percentage relative to the positive control (dHL-60 in presence of lysis buffer). Each bar represents the mean ± SEM of three independent experiments. Statistical analyses were performed by one-way analyses of Variance (ANOVA) followed by Newman-Keuls; *p < 0.05 from control group.