Fig. 5.

Ripk3 contributed to calcium overload via activating ER stress (n = 6/group). (A–B) Loss of Ripk3 reversed the upregulated GRP78 in response to IR injury as indicated by immunofluorescence. (C–F) The changes of ER stress markers, including PERK, CHOP and GRP78, in vivo were measured by Western blotting. (G–H) The changes of ER stress markers, including PERK, CHOP and GRP78, in vitro were measured by Western blotting. (I) The change in Ca²⁺ concentration was measured by flow cytometry. *P < 0.05 vs the sham group (in vivo) or WT group (in vitro), ^#P < 0.05 vs the WT+IR injury (in vivo) or WT+LPS+H₂O₂ group (in vitro).