Supplemental Fig. 1.

(A–C) Cardiac levels of IL-10, TNF-a and IL-6 by ELISA. (D) LDH release. (E) Cell viability was measured by MTT. (F–G) ROS content was measured by DHE staining. (H–J) The changes of GSH, SOD and MDA were detected by ELISA in cardiac tissue after IR injury. (K–L) The expression of Ripk3, PGAM5, p-MLKL and T-MLKL were measured by western blot. (M) More Ripk3 migrated on the ER under LPS/H₂O₂ in co-treatment in WT-cell, which was reversed by Ripk3 deletion. (N) The reactivation of ER stress under Ripk3 knockout re-upregulated the expression of CHOP by immunofluorescence in response to LPS and H₂O₂. *P < 0.05 vs WT group, ^#P < 0.05 vs the WT+LPS+H₂O₂ group. n = 6/group.