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. 2018 Mar 1;16:129–138. doi: 10.1016/j.redox.2018.02.022

Fig. 3.

Fig. 3

Knockout of LMP10 reduces Ang II-induced retinal oxidative stress and inflammation. A, Representative dihydroethydium (DHE) staining of central retinal sections (upper) and quantification of DHE intensity (lower) of wild-type (WT) and LMP10 knockout (KO) mice 3 weeks after saline or Ang II infusion (n = 6 mice per group). B, qPCR analysis of the mRNA levels of NOX1 and NOX4 in the retinas (n = 6 mice per group). C, Representative immunohistochemical staining of calcium-binding adapter molecule 1 (Iba1, arrow) in the retinas (upper) and quantification of Iba-positive cells (lower, n = 6 mice per group). Scale bar, 50 µm. D, qPCR analysis of the mRNA levels of IL-1β and IL-6 in the retinas (n = 3 mice per group). E, Immunoblotting analysis of the protein levels of LMP10, PTEN, p-AKT, AKT, p-IKKα/β, IKKα, IKKβ, p-IkBα, IkBα, p-p65 and p65 in the retinas. F, Quantification of the protein bands (n = 4 mice per group). GAPDH as an internal control. Data are the mean ± SEM. *P < 0.05, **P < 0.01 versus saline-infused WT mice; #P < 0.05, ##P < 0.01 versus Ang II-infused WT mice. IL-1β, interleukin-1β; IL-6, interleukin-6; NOX, NADPH oxidase. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; RPE, retinal pigment epithelium.