Fig. 3.

— Expression of human (Hs), N. vectensis (Nv), and T. adhaerens (Ta) cDNAs in HeLa and HEK293T cells. Equal amounts of pdcDNAMyc-(Hs/Nv/Ta)-Cdh1-Cyto, pdcDNAMyc-(Hs/Nv/Ta)-Ctnna2-Nterm and pdcDNAFlag-Ta-Pcasp plasmids were used to transfect HeLa or HEK293T cells. (A, C, E) Protein levels were analyzed by Western blotting using an anti-Myc antibody or an anti-Flag antibody as indicated; an anti-vinculin antibody was used as a control. (B, D, F) Expression of (Hs/Nv/Ta)-Cdh1-Cyto, (Hs/Nv/Ta)-Ctnna2-Nterm and Ta-Pcasp mRNA. After 24 h, total cellular RNA was isolated and analyzed by qRT-PCR. As negative controls, untransfected HeLa or HEK293T cells were used as well as transfection with empty pdcDNAMyc plasmid (mock). Values have been normalized such that the values of the different WT constructs of T. adhaerens are equal to 1. The results are representative of three experiments.