Table 2. Selected results for genetic optimization of the artificial Suzukiase for the synthesis of 2-methoxy-1,1′-binaphthyl 8b.

Entry	Temp [°C]	Complex	Protein	ee [%]	TON
1	RT	1	—	rac	20
2	RT	1	WT	58 (R)	78
3	RT	1	K121E	76 (R)	50
4	RT	1	K121D	34 (R)	7
5	RT	1	K121Q	74 (R)	20
6	RT	1	K121M	67 (R)	59
7	RT	1	K121F	67 (R)	38
8	RT	2	K121A	47 (S)	32
9	RT	2	S112M	44 (S)	53
10	RT	3	S112M	38 (R)	78
11	RT	3	S112A	36 (R)	52
12	RT	1	S112M	14 (S)	58
13	RT	1	S112A	60 (R)	58
14	RT	1	N118K–K121E	74 (R)	73
15	RT	1	N118S–K121E	76 (R)	79
16	RT	1	N118E–K121E	76 (R)	75
17	RT	1	N118L–K121E	72 (R)	86
18	RT	1	S112W–K121E	76 (R)	64
19	RT	1	S112N–K121E	69 (R)	61
20	RT	1	S112A–K121E	70 (R)	80
21	RT	1	S112Y–K121E	80 (R)	90
22	RT	1	S112Y–K121E	80 (R)	160 a
23	16	1	S112Y–K121E	84 (R)	50
24	4	1	S112Y–K121E	86 (R)	50 b
25	4	1	S112Y–K121E	90 (R)	50 c

^a0.50 mol% catalyst loading, 0.25 mol% Sav (tetramer) loading.

^b0.50 mol% catalyst loading, 0.25 mol% Sav (tetramer) loading, after 7 days.

^cPreparative scale (100 μmol). All reactions were performed in duplicate: Δee = ±1%, Δconv. = ±5%.