Skip to main content
. Author manuscript; available in PMC: 2018 May 15.
Published in final edited form as: Nat Protoc. 2016 Jun 23;11(7):1280–1298. doi: 10.1038/nprot.2016.067

Figure 1.

Figure 1

Proposed Wzy-dependent biosynthetic pathway of E. coli O157 LPS. The biosynthesis is initiated with the formation of GlcNAc-PP-Und, which is later epimerized to GalNAc-PP-Und54. Subsequently, the RU-PP-Und is assembled by three GTs (WbdN, WbdO and WbdP) and then translocated to the periplasmic face of the inner membrane by Wzx, where the repeating units are polymerized to form O-PS by Wzy in a block transfer mechanism that is regulated by Wzz. The last step includes WaaL catalyzed transfer of O-PS onto an individually synthesized Lipid A core structure to form the intact LPS.