Steps 25 and 102 |
Upon removing the solvent from purified undecaprenyl-phosphate, a waxy residue is formed which is difficult to transfer. |
This residue will take on a white powder form following the addition of 1–2 mL of hexanes, rotary evaporation, and drying under vacuum. This form greatly facilitates transfer of the product to another container should this process be necessary. |
Step 128 |
Starting material not completely consumed. |
Add another 100 µL of WbdN and incubate for 12 h at 37 °C. |
Step 142 |
Starting material not completely consumed. |
Add another 160 µL of WbdO, 40 µL of GDP-l-Fuc and incubate for 12 h at 37 °C |
Step 154 |
Starting material not completely consumed. |
Add another 60 µL of WbdP and incubate for 12 h at 37 °C. |