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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Nat Med. 2018 Mar 5;24(4):474–483. doi: 10.1038/nm.4505

Figure 2. Single-cell developmental classifier for BCP-ALL.

Figure 2

Healthy bone marrow aspirates from 5 donors were manually gated into 12 consecutive developmental stages of B lymphopoiesis (final gate is shown as a red box on a contour plot in the bottom, while the text above indicates prior gate(s) on lineage-negative cells; see Supplementary Figs. 1a and 1b for complete gating strategy). The mean arsinh-transformed expression of 11 proteins with relevance to normal B lymphopoiesis, shown in the heat-bar, was determined for each normal cell population (shown above the contour plots, where black indicates low expression and white – high expression). Single cells from each BCP-ALL sample were then assigned to their most similar normal population based on the shortest Mahalanobis distance calculated from expression of the same 11 proteins. Cells with distance above the classification threshold to all developmental populations remained unclassified (<1% for each patient). IgHi, intracellular immunoglobulin heavy chain; IgHs, surface immunoglobulin heavy chain.