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. Author manuscript; available in PMC: 2018 May 15.
Published in final edited form as: Leukemia. 2017 Nov 1;32(3):752–764. doi: 10.1038/leu.2017.316

Figure 3. Individual HLA-A24 antigen-specific CTL demonstrate proliferative response to HLA-A24+ multiple myeloma cells.

Figure 3

Figure 3

Figure 3

The proliferation of antigen-specific CTL was measured in response to HLA-A24+ or HLA-A24− myeloma cells in CFSE proliferation assays on day 5 or day 7 of culture by flow cytometry. The CTL generated by stimulation with an individual HLA-A24 peptide including XBP1 UN185-193 (Figure 3A), XBP1 SP223-231 (Supplemental Figure 3), CD138265-273 (Figure 3B) or CS1240-248 (Figure 3C) demonstrated time-dependent cell proliferation in response to HLA-A24+ KMS11 myeloma cells, but not to MHC mismatched HLA-A24− OPM1 myeloma cells. Background proliferation was determined using CFSE labeled individual antigen-specific CTL cultured in media alone.