Fig. 5.

Sod1 was abundantly expressed in astrocytes and protected astrocytes from oxidative damage. (A) Sod1 immunoreactivity co-localized with GFAP and was attenuated in AppTg/Cebpd^-/- mice compared with that in AppTg mice. The coronal sections of cortex tissue were subjected to immunofluorescence with anti-GFAP and anti-Sod1. (B) Quantitative analysis (n = 3 for each group) of Sod1-GFAP co-localization in AppTg and AppTg/Cebpd^−/− mice using ImageJ software. (C) The inhibition of Sod1 by TETA leads to cell death. Cell death was detected by the caspase3/7 activity kit using a fluorescent ELISA reader; data represent the two independent cell cultures analyzed in triplicate wells. n.s. not significant. (**p < 0.01, Student's t-test).