Fig. 6.

MsrA deletion does not increase oxidative stress in vascular smooth muscle cells or aorta. (A, B) Representative images (A) and quantification (B) of Cell ROX fluorescence (red) in MsrA-/- and WT VSMC. Nuclei: blue (TOPRO). Data were quantified as the fold-change relative to WT; n = 4 biological replicates. Scale bars 20 µm. (C) Superoxide levels in MsrA-/- and WT VSMC by L-012. Data were normalized to total protein; n = 4 biological replicates* p < 0.05 versus WT by two-tailed t-test. (D, E) Representative images (D) and summary data (E) of DHE staining (red) in aortic sections from MsrA-/- and WT mice; n = 11 biological replicates. Scale bars 50 µm. (F, G) Representative immunoblots (F) and summary data (G) for oxidized proteins in the indicated tissues from MsrA-/- and WT mice. Oxidized proteins were normalized to GAPDH (loading control); n = 4 biological replicates. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)