Fig. 7.

Apoptosis is decreased at baseline in MsrA-deficient vascular smooth muscle cells. (A, B) Representative dot plot (A) and summary data (B) of Annexin V by flow cytometry in MsrA-/- and WT VSMC. Data were quantified as the number of Annexin V positive cells relative to the total number of cells; n = 7–9 biological replicates. (C, D) Representative images (C) and summary data (D) TUNEL staining (red) in MsrA-/- and WT VSMC. Nuclei: blue (TOPRO); n = 5 biological replicates. Scale bars 50 µm. (E, F) Representative immunoblot (E) and summary data (F) for Bcl-2 expression in whole cell lysates from MsrA-/- and WT VSMC. Data were normalized to GAPDH; n = 6–7 biological replicates. (G) Oxidative stress-induced apoptosis by Annexin V staining (green) in MsrA-/- and WT VSMC treated with the indicated concentrations of H₂O₂ for 60 min (H) Summary data for G. n = 7–12 replicates. Nuclei: red (TOPRO). (B, D, F) * p < 0.05 versus WT by two-tailed t-test. (H) # p < 0.05 versus WT by multiple t-tests, one per row. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)