Fig. 1.

Effect of oxalate stimulation on the expressions of autophagy substrates in NRK-52E cells. Twenty-four hours after seeded, NRK-52E cells were incubated in the medium supplemented with 1% FBS for 1 h, followed by the addition of oxalate (0.75 mM) for indicated hours (A) or different concentrations of oxalate for 48 h (B), then the cells were harvested and the expressions of indicated proteins were detected by western blot and quantified using ImageJ software. *P < 0.05 and ** P < 0.01 versus untreated group.