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. 2018 Apr 2;16:414–425. doi: 10.1016/j.redox.2018.03.019

Fig. 2.

Fig. 2

Quantitative changes of autophagic vacuoles, autophagosomes and autolysosomes after exposure to urinary proteins in NRK-52E cells. (A) Quantitative changes of autophagic vacuoles under TEM in NRK-52E cells after treated with/without 0.75 mM oxalate for 48 h. The red arrows indicate autophagic vacuoles. Scale bar: 1 µm. (B) Fluorescent microscopic and quantitative analysis of NRK-52E cells that were transfected with pEGFP-LC3 plasmids and treated with/without 0.75 mM oxalate for 48 h. (C) Fluorescent microscopic and quantitative analysis of NRK-52E cells that were transfected with ptfLC3 plasmids and treated with/without 0.75 mM oxalate for 48 h. **P < 0.01 and ***P < 0.001.