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. 2018 Apr 2;16:414–425. doi: 10.1016/j.redox.2018.03.019

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© 2018 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Effects of rapamycin or chloroquine on autophagic activity and EG-induced CaOx crystals depositions in rat kidneys. (A) Quantitative changes in autophagic vacuoles under TEM in kidney sections from indicated rats. The red arrows indicate autophagic vacuoles. Scale bar: 1 µm. (B) The expressions of Beclin1 and SQSTM1 in kidney sections from indicated rats were detected by immunohistochemical staining and quantified using Image Pro Plus software. (magnification 200×) (C) Photomicrographs of kidney sections from indicated rats were obtained under dark field illumination with polarized light. Retained crystals exhibit strong birefringence (magnification 200×). The sizes of the areas of crystal deposition per field were estimated and quantified using ImageJ software. *P < 0.05, **P < 0.01 and ***P < 0.001.