Figure 3.

LOXL2 enhances proliferation and invasion capacities of glioma cells.

Notes: (A) Endogenous protein expression level of LOXL2 in normal cortical cell line (HCN-2) and 3 different glioma cell lines (U87-MG, U251, and U118-MG) was tested by Western blot. (B) Proliferation of U87-MG cells was tested by CCK-8 assay, revealing a higher cellular viability in cells o/e LOXL2. In contrast, cells transfected with LOXL2-siRNA showed a decreased proliferation pattern. Migration–Transwell (C) and Matrigel–Transwell (D) experiments were performed to evaluate the migration and invasion processes of glioma cells. Similar to the proliferation results, LOXL2 expression level was positively associated with cell migration and invasion capacities. (E) Immunoblotting results revealed that LOXL2 can upregulate the protein expression of Snail1, while downregulating E-cadherin. Besides, the phosphorylation status of Src and FAK proteins was also enhanced by LOXL2 overexpression. Western blot results were semi-quantified using ImageJ Software. All data were summarized from at least 3 independent experiments. *P<0.05 compared to CTL group.

Abbreviations: CTL, control; LOXL2, lysyl oxidase-like 2; NS, not significant; o/e, overexpressing.