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. 2018 May 8;9:951. doi: 10.3389/fimmu.2018.00951

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Copyright © 2018 Olaniyi, Pancotto, Grimaldi and Bagnoli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of human skin explant model. (A) The viability of human skin explant in culture. Each bar represents the reduction of PrestoBlue dye staining (a cell viability testing reagent) by skin samples at different time points in culture compared with freshly received skin tissue (time 0). Results are pooled from three independent experiments. (B) Immunofluorescence images showing skin cryosections of unstripped and stripped human skin tissue stained with anti-pan-cytokeratin antibody. Stripped skin lacks stratum corneum (arrow). Scale bar: 20 µm.