Skip to main content
. 2018 May 8;9:914. doi: 10.3389/fimmu.2018.00914

Table 1.

Primers used in real-time PCR for gene expression analysis.

Gene Primer sequence (5′–3′) Accession number Amplicon size Efficiency
pentraxin Fw: 5′-AGTTTTTGCTGCTGGTGGTG-3′
Rv: 5′-GCCAAAGAGAAAAGGACGTGG-3′
EU660933.1 199 1.95
lysozyme Fw: 5′-TGATGCAGGTTGTTGATGTTAATC-3′
Rv: 5′-TCCATCCCCCATATTGTAGGC-3′
KJ433681.1 194 1.94
transferrin Fw: 5′-GCCCCCAAACACAGATTCCT-3′
Rv: 5′-CCGTCAGCACCCATACTGTT-3′
FJ197144.1 177 1.95
hepcidin Fw: 5′-GGAATCGTGGAAGATGCCGT-3′
Rv: 5′-CAGACACCACATCCGCTCAT-3′
DQ131605.1 108 1.86
Interleukin (il)-1β Fw: 5′-ATCTGGAGGTGGTGGACAAA-3′
Rv: 5′-AGGGTGCTGATGTTCAAACC-3′
AJ269472.1 106 2.02
il-8 Fw: 5′-GTCTGAGAAGCCTGGGAGTG-3′
Rv: 5′-GCAATGGGAGTTAGCAGGAA-3′
AM490063.1 110 1.98
Chemokine (C–C motif) ligand 4 (ccl4) Fw: 5′-TCCTCGTCTCACTCTGTCTGT-3′
Rv: 5′-GACCTGCCACTGTCTTCAGC-3′
AM490064.1 197 1.95
il-10 Fw: 5′-CGACCAGCTCAAGAGTGATG-3′
Rv: 5′-AGAGGCTGCATGGTTTCTGT-3′
AM268529.1 199 2.06
Glucocorticoid receptor 1 (gr1) Fw: 5′-GAGATTTGGCAAGACCTTGACC-3′
Rv: 5′-ACCACACCAGGCGTACTGA-3′
AY549305 401 1.92
Glucocorticoid receptor 2 (gr2) Fw: 5′-GACGCAGACCTCCACTACATTC-3′
Rv: 5′-GCCGTTCATACTCTCAACCAC-3′
AY619996 403 1.97
Heat shock protein 70 (hsp70) Fw: 5′-GCTCCACTCGTATCCCCAAG-3′
Rv: 5′-ACATCCAGAAGCAGCAGGTC-3′
AY423555.2 172 1.94
Ribosomal protein l13 (rpl13) Fw: 5′-AAGGGAGACAGCACTGAGGA-3′
Rv: 5′-TGCCAAAAAGACGAGCGTTG-3′
DQ836931.1 175 2.00
Elongation factor 1α (ef1α) Fw: 5′-AACTTCAACGCCCAGGTCAT-3′
Rv: 5′-CTTCTTGCCAGAACGACGGT-3′
AJ866727.1 144 1.97
40S ribosomal protein SA (rpsa) Fw: 5′-TGATTGTGACAGACCCTCGTG-3′
Rv: 5′-CACAGAGCAATGGTGGGGAT-3′
HE978789.1 79 1.93