Table 1.
Gene | Primer sequence (5′–3′) | Accession number | Amplicon size | Efficiency |
---|---|---|---|---|
pentraxin | Fw: 5′-AGTTTTTGCTGCTGGTGGTG-3′ Rv: 5′-GCCAAAGAGAAAAGGACGTGG-3′ |
EU660933.1 | 199 | 1.95 |
lysozyme | Fw: 5′-TGATGCAGGTTGTTGATGTTAATC-3′ Rv: 5′-TCCATCCCCCATATTGTAGGC-3′ |
KJ433681.1 | 194 | 1.94 |
transferrin | Fw: 5′-GCCCCCAAACACAGATTCCT-3′ Rv: 5′-CCGTCAGCACCCATACTGTT-3′ |
FJ197144.1 | 177 | 1.95 |
hepcidin | Fw: 5′-GGAATCGTGGAAGATGCCGT-3′ Rv: 5′-CAGACACCACATCCGCTCAT-3′ |
DQ131605.1 | 108 | 1.86 |
Interleukin (il)-1β | Fw: 5′-ATCTGGAGGTGGTGGACAAA-3′ Rv: 5′-AGGGTGCTGATGTTCAAACC-3′ |
AJ269472.1 | 106 | 2.02 |
il-8 | Fw: 5′-GTCTGAGAAGCCTGGGAGTG-3′ Rv: 5′-GCAATGGGAGTTAGCAGGAA-3′ |
AM490063.1 | 110 | 1.98 |
Chemokine (C–C motif) ligand 4 (ccl4) | Fw: 5′-TCCTCGTCTCACTCTGTCTGT-3′ Rv: 5′-GACCTGCCACTGTCTTCAGC-3′ |
AM490064.1 | 197 | 1.95 |
il-10 | Fw: 5′-CGACCAGCTCAAGAGTGATG-3′ Rv: 5′-AGAGGCTGCATGGTTTCTGT-3′ |
AM268529.1 | 199 | 2.06 |
Glucocorticoid receptor 1 (gr1) | Fw: 5′-GAGATTTGGCAAGACCTTGACC-3′ Rv: 5′-ACCACACCAGGCGTACTGA-3′ |
AY549305 | 401 | 1.92 |
Glucocorticoid receptor 2 (gr2) | Fw: 5′-GACGCAGACCTCCACTACATTC-3′ Rv: 5′-GCCGTTCATACTCTCAACCAC-3′ |
AY619996 | 403 | 1.97 |
Heat shock protein 70 (hsp70) | Fw: 5′-GCTCCACTCGTATCCCCAAG-3′ Rv: 5′-ACATCCAGAAGCAGCAGGTC-3′ |
AY423555.2 | 172 | 1.94 |
Ribosomal protein l13 (rpl13) | Fw: 5′-AAGGGAGACAGCACTGAGGA-3′ Rv: 5′-TGCCAAAAAGACGAGCGTTG-3′ |
DQ836931.1 | 175 | 2.00 |
Elongation factor 1α (ef1α) | Fw: 5′-AACTTCAACGCCCAGGTCAT-3′ Rv: 5′-CTTCTTGCCAGAACGACGGT-3′ |
AJ866727.1 | 144 | 1.97 |
40S ribosomal protein SA (rpsa) | Fw: 5′-TGATTGTGACAGACCCTCGTG-3′ Rv: 5′-CACAGAGCAATGGTGGGGAT-3′ |
HE978789.1 | 79 | 1.93 |