Figure 3.

Motility phenotype of the fcpB⁻ mutant in semisolid media. Soft agar plates were inoculated with 10⁵ leptospires of the wild-type, fcpB⁻ mutant, and fcpB^−/+ complemented strains. (A) The diameter of the zone of spread for each colony was measured using image analysis software. Bar scale, 10 mm. (B) The average colony diameter measured for the fcpB⁻ mutant was significantly smaller (p < 0.0001) than for the wild-type. The difference in average colony diameter between the fcpB^−/+ complemented and wild-type strains was not significant.