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. 2018 May 8;11:148. doi: 10.3389/fnmol.2018.00148

FIGURE 4.

FIGURE 4

Application of L-lactate and 3Cl-5OH-BA decreases [glucose]i in astrocytes. (A,B) Representative (above) and mean time-course (below) of FLII12Pglu-700 μδ6 FRET ratio signal, reporting [glucose]i, upon the addition of (A) L-lactate (20 mM) and (B) 3Cl-5OH-BA (0.5 mM). Data are expressed as the percentages of the FRET ratio signals (EYFP/ECFP) relative to the baseline ratio values. The initial rate of change in [glucose]i was determined by fitting the regression lines to the signal decreases; ΔFRET (%) = –k (%/min) × Δt (min), where t is the time and the k–slope is the initial ΔFRET decline: k(A,above) = 4.94 ± 0.28 %/min, k(A,below) = 2.90 ± 0.36 %/min, and k(B,above) = 2.32 ± 0.29 %/min, k(B,below) = 1.05 ± 0.11 %/min. Note that the addition of L-lactate transiently decreased the FRET ratio, while the 3Cl-5OH-BA persistently decreased the FRET ratio. Each data point represents the mean ± s.e.m. (C) Mean changes in the FRET ratio (ΔFRET ratio; left) and initial rates in the FRET ratio decrease (ΔFRET/Δt; right) upon L-lactate and 3Cl-5OH-BA stimulation. Numbers adjacent to the bars depict the number of cells analyzed. Data shown are in the format of the mean ± s.e.m (∗P < 0.05, ∗∗∗P < 0.001). Data for every set of experiment was acquired from at least two different animals.