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. 2018 Mar 12;98(5):1303–1312. doi: 10.4269/ajtmh.17-0214

Figure 1.

Figure 1.

Detection limit of Histoplasma capsulatum DNA and specificity test of the Hc100 nested polymerase chain reaction (PCR). (A) Application of the Hc100 nested PCR with DNA extracted from the yeast phase of H. capsulatum diluted from 20 to 0.1 pg/μL. (B) Amplification products of the Hc100 nested PCR from the 1:10, 1:100, and 1:1,000 serial dilutions of compost infected with 3,000 CFU/mL of H. capsulatum in the mycelial phase. (C) In the specificity test the amplification product was obtained only from the Histoplasma spp. DNA sample. The observed bands were due to the excess DNA used and was corrected when the DNA concentration was controlled (e.g., the positive control with 2 ng/μL DNA). Line: 1 Paracoccidioides brasiliensis (Pb) 01, 2: Pb03, 3: Pb18, 4: Pb60855, 5: Coccidioides immitis, 6: Blastomyces dermatitidis, 7: Emmonsia crecens, 8: Emmonsia parva, 9: Sporothryx schenckii, 10: Colletotrichum spp., 11: Trichoderma spp., 12: Pseudocercospora fijiensis, 13: Aspergillus spp., 14: Chrysosporium spp., 15: Microsporum gypseum, 16: Geotrichum spp., 17: Fusarium spp., 18: Penicillium spp., 19: Saccharomyces cerevisiae, 20: Candida albicans, 21: Cryptococcus neoformans, 22: Histoplasma duboisii, 23: H. capsulatum, 24: negative control.