Table 1.
Diagnostic techniques and samples studied pre- and posttreatment with Ambisome (liposomal amphotericin B)
| Biopsy | FPLI | Buffy coat | Blood serum | |||
|---|---|---|---|---|---|---|
| Pre-T | Pre-T | Pre-T | Post-T | Pre-T | Post-T | |
| Culture* | Positive | – | Growth not detected | – | – | – |
| PCR (Ct) | – | Positive (Ct 24) | Positive (Ct 39) | Negative (Ct 45) | – | – |
| WB (kDa) | – | – | – | – | 14, 16, 24, 28, 30, 46, 65, 70 | 14, 16, 30, 46, 65, 70 |
| ELISA (units) | – | – | – | – | 170 U | 35 U |
Ct = threshold cycle; ELISA = enzyme-linked immunosorbent assay; FPLI = filter paper lesion impression; Post-T = posttreatment; Pre-T = pretreatment; WB = Western blot.
*
Three months monitoring.