Table 3.
Operative capabilities of the LAMP test compared with microscopy and qPCR
| Smears | Sandflies | ||
|---|---|---|---|
| Reference test | Microscopy % (95% CI) | qPCR % (95% CI) | qPCR % (95% CI) |
| Sensitivity | 100 (98.6–100.0) | 100 (98.7–100.0) | 100 (97.5–100.0) |
| Specificity | 71.4 (44.2–98.7) | 90.9 (69.4–100.0) | 96.8 (88.9–100.0) |
| PPV | 90.0 (79.5–100.0) | 97.5 (91.4–100.0) | 95.2 (83.8–100.0) |
| NPV | 100.0 (95.0–100.0) | 100.0 (95.0–100.0) | 100.0 (98.3–100.0) |
| LR (+) | 3.5 (1.5–8.0) | 11.0 (1.7–71.3) | 31.0 (4.5–213.8) |
| LR (−) | – | – | – |
LAMP = loop-mediated isothermal amplification; LR = likelihood ratio; NPV = negative predictive value; PPV = positive predictive value; qPCR = real-time polymerase chain reaction. The estimator could not be calculated because one of the fields contained no data during the dispersion analysis.