Figure 1.
Representative melt curve profiles from two different skin snip DNA samples that displayed non-single peak profiles indicative of mixed template DNA. Species-specific qPCR results for these samples indicated the presence of both Mansonella perstans and Onchocerca volvulus. The Tm range for O. volvulus and Mansonella spp.–positive controls is indicated with checkered gray and solid gray bars, respectively. The melt curve profile from each skin snip sample is shown in black (duplicate replicates in solid vs. dashed line) (A) melt curve profile with a main peak and a secondary smaller peak. (B) Melt curve profile with two distinct peaks.