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. 2018 Mar 19;98(5):1374–1381. doi: 10.4269/ajtmh.17-0857

Figure 2.

Figure 2.

Gel electrophoresis analysis of Plasmodium vivax–specific (A) loop-mediated isothermal amplification products. L = 100-bp molecular marker, NC = negative control (nuclease-free water), and lanes 1–9 = P. vivax–positive clinical isolates. (B) 18SrRNA-specific multiplex nested polymerase chain reaction (nPCR) products. Lane L = 100-bp DNA ladder, NC = negative control (nuclease-free water), PC = positive control (for P. vivax patient sample, 117 bp and for P. falciparum-3D7, 205 bp), and lanes 1–9 = P. vivax–positive species-specific multiplex nPCR products. (C) Real-time polymerase chain reaction results showing melting curve analysis for P. vivax clinical isolates. (D) Standard curve (y = −3.337x + 34.331), where y axis shows the cycle threshold. This figure appears in color at www.ajtmh.org.