(A) Design for treatment groups showing maternal diet (E10.5-PN21) and post-weaning diet (PN21-week 14). Maternal VAD diet is based on LF12. HFD-VAD is a VA deficient equivalent to HFD. Male mice from at least two litters were used; number (n) is shown per group. (B) Serum retinol, liver retinol, and liver retinyl ester for individual mice in each group. (C) Body weight (D) Epididymal fat pad weight (E) Correlation between fat and body weight among BD-HFD, LF12-HFD, and GVAD-HFD groups (F) Liver weight. Data are mean±SEM *p-value<0.05, **p-value<0.01