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. 2018 May 15;13(5):e0196976. doi: 10.1371/journal.pone.0196976

Fig 2. Two consecutive challenges of LPS result in microvascular responses in organs comparable to those induced by a single LPS challenge.

Fig 2

Mice were challenged with LPS by one single i.p. injection (2mg/kg) or two consecutive injections (1mg/kg per injection), with the second injection 3h after the first challenge. 24h after the initial challenge, mRNA expression of endothelial pro-inflammatory molecules, vascular integrity related molecules, blood flow-sensitive transcription factor KLF2 in kidney (A), lungs (B), and liver (C), and kidney damage related markers NGAL and Kim1 (A) were determined by real time RT-PCR using GAPDH as housekeeping gene. All data are presented as fold change relative to saline control. Values are shown as mean ± SD (n = 6 per group). *. P<0.05, 1xLPS respectively 2xLPS vs. control; #, P<0.05, 2xLPS vs. 1xLPS.