Fig 1. Validation of the V. vinifera PEP internal control in the duplex real-time RT-PCR GPGV assay.

Three replicates of a GPGV infected plant extract were ten-fold serially diluted with healthy plant extract. Amplification plots by GPGV singleplex (blue) and GPGV-PEP duplex (red) real time RT-PCR assays are shown. Two negative controls (black) are included.