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. 2018 May 15;13(5):e0197237. doi: 10.1371/journal.pone.0197237

Table 1. Sensitivity comparison between TaqMan and SYBR Green GPGV real-time RT-PCR detection methods.

Serial dilutionsa
qPCR method Targeted region Undiluted extract 10−1 10−2 10−3 10−4 10−5 10−6 10−7
TaqMan
(this study)
MP/CP + + + + + - - -
SYBR Green
(Bertazzon et al. [4])
RdRp + + + - - - - -
CP + + + - - - - -

a Ten-fold serial dilutions of GPGV infected plant material with healthy plant extracts