Table 1. Sensitivity comparison between TaqMan and SYBR Green GPGV real-time RT-PCR detection methods.
Serial dilutionsa | |||||||||
---|---|---|---|---|---|---|---|---|---|
qPCR method | Targeted region | Undiluted extract | 10−1 | 10−2 | 10−3 | 10−4 | 10−5 | 10−6 | 10−7 |
TaqMan (this study) |
MP/CP | + | + | + | + | + | - | - | - |
SYBR Green (Bertazzon et al. [4]) |
RdRp | + | + | + | - | - | - | - | - |
CP | + | + | + | - | - | - | - | - |
a Ten-fold serial dilutions of GPGV infected plant material with healthy plant extracts