Table 2. Real-time RT-PCR cycle threshold (Ct) values for GPGV and PEP internal control, absolute quantitation, symptomatology and presence of other grapevine viruses in GPGV positive samples collected from D.O. Utiel-Requena.
| Sample | Variety | Ct Values (GPGVa) | Viral titer | Ct Values (PEP) | Symptomsb | Other virusesc |
|---|---|---|---|---|---|---|
| RQ25 | Bobal | 25.0 | 1.7 x 105 | 20.2 | CM, LD | GFLV, GLRaV-3 |
| RQ30 | Bobal | 23.4 | 3.9x 105 | 21.6 | CM, LD | GFkV, GFLV, GLRaV-3 |
| RQ99 | Macabeo | 21.2 | 1.3 x106 | 21.3 | CM | GFLV, GFkV, GLRaV-2 |
| RQ106 | Macabeo | 23.1 | 4.5 x105 | 20.6 | SL | GLRaV-2 |
| RQ108 | Macabeo | 23.2 | 4.4 x 105 | 21.5 | CM | GFLV |
| RQ109 | Macabeo | 23.2 | 4.4 x 105 | 18.7 | CM | GFLV |
| RQ110 | Macabeo | 23.2 | 4.4 x 105 | 19.7 | CM | GFLV |
| RQ111 | Tintorera | 22.9 | 5.2 x 105 | 21.7 | CM | GFLV |
| RQ113 | Tintorera | 20.5 | 1.8 x 106 | 21.6 | CM | GFLV |
| RQ115 | Tintorera | 24.7 | 2.0 x 105 | 21.9 | CM | GFLV |
| RQ116 | Bobal | 26.6 | 7.5 x 104 | 22.0 | CM | - |
| RQ117 | Bobal | 18.5 | 1.1 x 108 | 21.9 | CM | - |
| RQ118 | Bobal | 27.5 | 4.6 x 104 | 22.8 | CM | - |
| RQ121 | Tintorera | 32.3 | 3.6 x 103 | 22.7 | SL | GLRaV-3, GFkV |
| RQ124 | Tintorera | 30.9 | 7.8 x 103 | 23.5 | SL | GFLV |
| RQ125 | Tintorera | 23.2 | 4.3 x 105 | 22.7 | SL | GFLV, GLRaV-2 |
| RQ136 | Bobal | 24.2 | 2.6 x 105 | 20.3 | CM | GFLV |
| RQ140 | Bobal | 31.5 | 5.7 x 103 | 20.7 | CM | GFLV |
| RQ142 | Bobal | 32.6 | 3.2 x 103 | 20.5 | SL | GLRaV-2, GLRaV-3 |
| RQ143 | Bobal | 33.8 | 1.6 x 103 | 21.0 | CM | GLRaV-2, GLRaV-3 |
| RQ145 | Macabeo | 32.0 | 4.2 x 103 | 20.5 | CM | GFLV |
| RQ150 | Macabeo | 33.2 | 2.3 x 103 | 20.7 | CM | GFLV |
| RQ151 | Macabeo | 33.0 | 2.6 x 103 | 20.8 | CM | GFLV |
| RQ160 | Tintorera | 32.7 | 3.0 x 103 | 24.0 | CM | GFLV |
a Grapevine Pinot gris virus (GPGV)
bChlorotic mottling (CM); Leaf deformation (LD); Symptomless (SL).
cGrapevine fanleaf virus (GFLV); Grapevine leafroll-associated virus 2 (GLRaV-2); Grapevine leafroll-associated virus 3 (GLRaV-3); Grapevine fleck virus (GFkV).