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. 2018 May 3;14(5):e1007366. doi: 10.1371/journal.pgen.1007366

Fig 2. Inhibition of calcineurin signaling induces the expression of pro-inflammatory cytokines in keratinocytes.

Fig 2

A-D: qRT-PCR analysis of TNFα, IL-8 and CXCL1 expression; results were normalized to levels of 36B4 mRNA. A. Total mRNA isolated from mouse ears from the experiments shown in Fig 1A; B. Total mRNA extracted from primary MKCs from CnB1fflox/flox mice infected with either Ad-Cre or Ad-GFP viruses, with or without UVB treatment; C. Total mRNA isolated from TPA-treated HKCs transfected with siRNA of CnB1 (siCnB1) or a control scramble siRNA (siCtrl) at 24 hr; D. Total mRNA from HKCs treated with CsA or DSMO (Ctrl) for 24 hr, followed by treatment with TPA for another 24 hr. E-G: ELISA analysis of indicated cytokine protein levels in the medium. E. Conditioned medium collected from MKC cultures shown in B; F,G. Conditioned medium collected from HKC cultures shown in C. Student’s t-test was used for statistical analysis in A-G, standard error bars provided, * p<0.05, **p<0.01, n = 3.