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. 2018 May 3;14(5):e1007366. doi: 10.1371/journal.pgen.1007366

Fig 4. TTP negatively regulates cytokine production in keratinocytes.

Fig 4

A. HKCs were transfected with 3 individual siRNAs of TTP (siTTP-1, -2, -3), and 72 hr after transfection, cells were analyzed for TTP and cytokines (TNFα, IL-8, CXCL1) expression by qRT-PCR; expression levels were normalized against the 36B4 gene. * p<0.05, ** p<0.01 when comparing the TTP knockdown group with the control group (siCtrl). B. Cells analyzed by immunoblot for TTP protein level with tubulin as a loading control. The image of full-blot of B is shown in S7 Fig; C. HKCs were infected with a TTP expressing lentivirus (TTP-OE); 72 hr after infection, cells were analyzed for TTP mRNA by qRT-PCR. D. TTP protein levels analyzed by immune-blotting. E. Cytokine expression analyzed by qRT-PCR. ** indicates p<0.01 when comparing cells in the TTP-OE group to the control group (Ctrl). F,G. Conditioned media were collected from the TTP over-expressing cells cultured for 72 hr for analysis of IL-8 (F) and TNFα (G) level by ELISA. *: p<0.05 and **:p<0.01. All experiments were repeated 3 times.