Fig 5. Knockdown of Tsg101 and Alix decrease the production of infectious OROV.

HeLa cells were transfected with control, Tsg101 or Alix siRNAs and infected with OROV (MOI = 1). (A, B) After 18 h p.i, supernatant of infected cells were harvested and processed for PFU analysis (upper panels), and cell lysate were collected for western blot assay (lower panels). Supernatant samples were equalized according to the total amount of proteins in cell lysates. Two different siRNA sequences were used to deplete Tsg101 (A) or Alix (B) as indicated. Bars represent the relative mean ± SEM (n = 4 for panel A, and n = 3 for panel B). *, P < 0.05 **, P < 0.005 (one-way ANOVA followed by Bonferroni’s post-test).