Table 1. Primers and constructs for heterologous expression of Cgr proteins in R. erythropolis.

Restriction sites are bolded.

Construct	For/	Sequence	Restriction sites	Vector	Anneal temp (°C)	Extend time (s)
	Rev
cgr operon	For	ACTGACCCATGGATGGAATACGGAAAGTGCC	n/a	n/a	71	75
	Rev	GTTTTACTGCAGTTACGCCGCCGTCGAA
Cgr1 + Cgr2	For	TGACGAATTCTAATGGAATACGGAAAGTGCCG	EcoRI,	pTipQT2	70	90
	Rev	TTATAAGATCTCGCCGCCGTCGAAAG	BglII
Cgr1	For	TCGAACATATGATGGCTGAGGAACCTGTGG	NdeI,	pTipQT1	65	60
	Rev	ATAACTCGAGTCACGCCGCCGTCGAAA	XhoI
Cgr2 (native)	For	ACTGACCCATGGGCATGGAATACGGAAAGTGCC	NcoI, HindIII	pTipQC2	65	60
	Rev	ATTAGAAGCTTTCACTCCCACGGCTCGAG
Cgr2-CHis6 (native)	For	ACTGACCCATGGGCATGGAATACGGAAAGTGCC	NcoI, HindIII	pTipQC1	65	60
	Rev	GTTAGAAGCTTCTCCCACGGCTCGAG
Cgr2(−48aa)-NHis6	For	TATTACCATGGATCAGACCGCGCCTGC	NcoI, HindIII	pTipQC2	65	60
	Rev	ATACTAAGCTTCTCCCACGGCTCGA
Cgr2(−48aa)-CHis6	For	TATTACCATGGATCAGACCGCGCCTGC	NcoI, HindIII	pTipQC1	65	60
	Rev	ATACTAAGCTTTTACTCCCACGGCTCGA
Sequencing primers	For	CGTGGCACGCGGAAC	n/a	All pTip vectors	n/a	n/a
	Rev	GTGCAGGTTTCGCGTG