Table 2. Primers for site-directed mutagenesis of Cgr2.

Amino acid numbering is based on full length Cgr2 sequence. Introduced mutations are bolded.

Mutant	F/R	Sequence
C82A	For	CAGCGGCGGCACGGCCGCGGCCATCG
	Rev	CCTCGATGGCCGCGGCCGTGCCGCCG
C111A	For	GCGGCAACTCGGCACTAGCCGGTGGATACAT
	Rev	CCAGCATGTATCCACCGGCTAGTGCCGAGTTG
C158A	For	ATATGATCCGCGAGGCGGCCTTGCGCTCCGGC
	Rev	GCCTCGCCGGAGCGCAAGGCCGCCTCGCGGAT
C187A	For	GCCCCCGGTCTGGTCAGCCGGCGACACGG
	Rev	GGCCTCCGTGTCGCCGGCTGACCAGACCGG
C231A	For	CGAAATCGAGATGGGCGCCGAGGTGGCGCAC
	Rev	GATGTGCGCCACCTCGGCGCCCATCTCGAT
C265A	For	GGCGTGGTCATGGCGGCCGCTTCGGTGGA
	Rev	GTTGTCCACCGAAGCGGCCGCCATGACCA
C321A	For	GATCGGTGCTGAGCTTGCCATGCAGCAGGC
	Rev	CACGGCCTGCTGCATGGCAAGCTCAGCACC
C327A	For	CATGCAGCAGGCCGTGGCCATGAACGATTCT
	Rev	GATAGAATCGTTCATGGCCACGGCCTGCTG
C371A	For	GACCGGCAGACGGTTTGCCCAGGACGATGCCG
	Rev	CTCGGCATCGTCCTGGGCAAACCGTCTGCC
C384A	For	CTATGTCATGCACGAGGCCGCGCAAGCTGCA
	Rev	CCATGCAGCTTGCGCGGCCTCGTGCATGAC
C425A	For	CATACGCCCGACACGGCCGATACTACGTTC
	Rev	CGAGAACGTAGTATCGGCCGTGTCGGGCGT
C443A	For	GCCGAGTTTATCGGCGCCGATCCGACCGC
	Rev	GAGGGCGGTCGGATCGGCGCCGATAAACTC
C459A	For	GAGGTGGAACTCTTTCGCCGAGGCCGGTTTG
	Rev	CATCCAAACCGGCCTCGGCGAAAGAGTTCCA
C483A	For	GACGCCGCCGTTCTACGCCGATGTCGTGCGC
	Rev	GGGGCGCACGACATCGGCGTAGAACGGCGG
C521A	For	CTGTACGGCGCCGGGGCCATCATCGGGGGT
	Rev	GTTACCCCCGATGATGGCCCCGGCGCCGTA
C535A	For	GCCTTCTACTTCGGCGCCGGCTGGTCCATC
	Rev	CGTGATGGACCAGCCGGCGCCGAAGTAGAA
Y333N	For	GCATGAACGATTCTATCAACGTAGGCGGCATCA
	Rev	TCGCTGATGCCGCCTACGTTGATAGAATCGTTCA
Y532F	For	GATGCCGAGTGGGGCTTTGTCATGCACG
	Rev	GCACTCGTGCATGACAAAGCCCCACTCG
G536A	For	TTCTACTTCGGCTGCGCCTGGTCCATCA
	Rev	GTTCGTGATGGACCAGGCGCAGCCGAAG