Table 1.
Primer sequences.
| Gene | Primer Sequence 5′ → 3′ | Function |
|---|---|---|
| β-Actin | F: TTCCTTCCTGGGTATGGAAT R: GAGGAGCAATGATCTTGATC |
Cytoskeletal protein (Housekeeping gene) |
| CD200 | F: CTCTCTATGTACAGCCCATAG R: GGGAGTGACTCTCAGTACTAT |
Neuronal antigen that binds CD200R to inhibit microglia |
| CD200R | F: TAGAGGGGGTGACCAATTAT R: TACATTTTCTGCAGCCACTG |
Cognate receptor for CD200 |
| CX3CL1 | F: ATCATCCTGGAGACGAGACAGC R: CCACACGCTTCTCAAACTTGCC |
Neuronal antigen that binds CX3CR1 to inhibit microglia |
| CX3CR1 | F: TCAGGACCTCACCATGCCTA R: CGAACGTGAAGACAAGGGAG |
Cognate receptor for CX3CL1 |
| IL-1β | F: CCTTGTGCAAGTGTCTGAAG R: GGGCTTGGAAGCAATCCTTA |
Pro-inflammatory cytokine |
| IL-6 | F: AGAAAAGAGTTGTGCAATGGCA R: GGCAAATTTCCTGGTTATATCC |
Pro-inflammatory cytokine |
| TNFα | F: CAAGGAGGAGAAGTTCCCA R: TTGGTGGTTTGCTACGACG |
Pro-inflammatory cytokine |