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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Brain Behav Immun. 2018 Mar 15;70:21–35. doi: 10.1016/j.bbi.2018.03.020

Fig. 1.

Fig. 1

Primary human macrophages express low but variable levels of both the dopamine transporter and tyrosine hydroxylase. Peripheral blood mononuclear cells (PBMC) were isolated from human blood by Ficoll gradient centrifugation. Monocytes were isolated from the PBMC by adherence, and matured into monocyte-derived macrophages (MDM) through 6 days of culture in the presence of M-CSF (10 ng/mL). MDM RNA was isolated, converted into cDNA and analyzed for expression of DAT and TH by quantitative reverse transcriptase PCR (qRT-PCR). We utilized Taqman primers for the dopamine transporter (DAT) and tyrosine hydroxylase (TH) designed by Applied Biosystems. Both DAT and TH transcripts were found in all human samples probed (n = 15). All samples were normalized to an 18S control primer. Data were transformed using the equation 2−ΔCt and are expressed relative to the expression of 18S in each donor.