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mCP displays Ca(II)-dependent heterotetramer formation. Analytical SEC chromatograms of 100 μM mCP in the absence (thick black trace) and presence of 1, 2, 5, 10, and 25 mM (thick red trace) Ca(II) (75 mM HEPES, 100 mM NaCl, pH 7.0, 200 μM TCEP) at 4 °C. Elution volumes and corresponding molecular weights are given in Table S3.
